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| HDRI - High Dynamic Range Image Acquisition |
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In fluorescence microscopy in particular you will often find that your camera is unable to acquire all the contrast information in your specimens. High Dynamic Range Imaging or abbreviated HDRI allows you to capture details from strongly fluorescent locations together with structures from weakly fluorescent positions in a single image.
The HDR Image Acquisition module generates an image with an increased dynamic range from several pictures with different exposure times. This means that with applied HDRI rendering additional information is made available. You can use this extra data to achieve more precise and even more meaningful measurement results. At the same time, you benefit from enhanced high end image quality, as high dynamic range acquisition technique results in reduced image noise.
The HDRI Acqusition module at a glance
Up to now, if you wanted to pick out structures in bright regions, you had to accept that dark areas would be underexposed:
If, on the other hand, you captured the details in dark positions and, consequently, increased the exposure time, bright positions would be overexposed:
Using high end HDRI your resulting image contains the details from both dark and bright image regions:
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| Fluorescence high dynamic range image of a rat brain, acquired using the AxioCam MRm |
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