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Would you like to measure FISH signals in multichannel fluorescence images in order to localize DNA or RNA sequences?
AxioVision QuantiFISH automatically converts an image containing fluorescence signals in several focus planes into an image with extended focus. In this image all cell nuclei and FISH signals are present in a single plane. In the DAPI-stained interphase cell nuclei you can then automatically determine the FISH signals for each nucleus and each fluorescence channel.
The measurement results in the data table can be compared directly with the original image, image with extended focus and signal image, which are arranged next to each other.
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| A z-plane containing fluorescence-labeled FISH signals. |
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| All z-planes are combined to form an image with extended focus. This contains all the FISH signals in the cell nuclei concerned. |
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| In the resulting image, the number of FISH signals in the individual fluorescence channels is marked in each cell nucleus. |
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