| Optical Sectioning Using Structured Illumination |
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Increase contrast and resolution, even when viewing whole embryos – quickly, easily and at affordable cost.
 ApoTome.2: The Module
Axio Observer with ApoTome.2
Insert ApoTome.2 into the beam path.
ApoTome.2: Grid positions
ApoTome.2: Modules for Axio Imager.2 and Axio Observer
Neuronal tissue - supra-optical nucleus of a rat
Objective: Plan-Apochromat 63x/1.4
2-channel fluorescence
Green: Neurophysin II (vasopressin-expressing neurons)
Red: VGLUT2 (presynaptic terminals)
Data: Courtesy Dr. K. Mueller,
University of Cincinnati, USA
Embryo of a zebra fish
Objective: Plan-Neofluar 10x/0.3
Green: Neuron
Red: Cell adhesion molecule NCAM
Data: Courtesy Dr. Martin Bastmeier and
Dr. Monika Marx, University of Jena
Section through the small intestine of a mouse
Objective: Plan-Apochromat 63x/1.4
3-channel fluorescence
Green: Cell nuclei
Red: F-actin
Blue: Glycocalix
When you observe fluorescence-labeled specimens in 3D, specimen details outside the depth-of-field of the objective appear blurred. This reduces the signal-to-noise ratio in your data. ApoTome.2 solves this problem by structured illumination.
ApoTome.2 is a module with integrated grid structures. Insert the module into your microscope and leave AxioVision to do the rest for you. Taking account of the objective used, AxioVision automatically places the optimal grid into the microscope's beam path – no manual change is required.
As an excitation illuminant, use the Colibri.2 LED light source or any conventional white light source. Contrary to confocal systems laser excitation is not needed.
ApoTome.2 works with, and can be retrofitted, to all partially motorized Axio Imager and Axio Observer microscopes.
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