Recent Literature Recent Literature
Flatworms Peter Reddien and colleagues investigated how flatworms can regenerate themselves from small body fragments. See their images of flatworms and cell lineage studies using the SteREO Lumar as well as the Apotome and LSM 700. One of their images is also featured on the journal cover.

Wagner, D.E. et al. Clonogenic neoblasts are pluripotent adult stem cells that underlie planarian regeneration. Science 332:811-6 (2011).
Nature Taming Complexity Michael Potente and colleagues used the LSM 710 to acquire images of blood vessel growth in human umbilical vein endothelial cells. This work supported their study of Notch signaling in cell specification and tissue patterning.

Guarani, V. et al. Acetylation-dependent regulation of endothelial Notch signalling by SIRT1 deacetylase. Nature 473:234-8 (2011).
Nature Taming Complexity Susan Ferro-Novick and colleagues investigated how intracellular vesicle traffic achieves directionality. See images of yeast and mammalian cells acquired using the Cell Observer SD.

Lord, C. et al. Sequential interactions with Sec23 control the direction of vesicle traffic. Nature 473:181-6 (2011).
mechanisms of temporal contro Federico Calegari and colleagues used the ApoTome to image mouse hippocampus and investigate mechanisms of temporal control of neurogenesis in the mammalian brain.

Artegiani, B. et al. Overexpression of cdk4 and cyclin D1 triggers greater expansion of neural stem cells in the adult mouse brain. J Exp Med. 208:937-48 (2011).
Inhibitory Mechanisms David Sansom and colleagues investigated inhibitory mechanisms behind T-cell immune responses. See images and live cell movies using the LSM 780.

Qureshi, O.S. et al. Trans-endocytosis of CD80 and CD86: a molecular basis for the cell-extrinsic function of CTLA-4. Science. 332:600-3 (2011).
Nature Pat Levitt and colleagues used the
AxioCam HRc to acquire images to support their study of fetal brain development.

Bonnin, A. et al. A transient placental source of serotonin for the fetal forebrain. Nature. 472:347-50 (2011).
Nature Edith Heard and colleagues investigated the different mechanisms used by mammals to control X-chromosome inactivation. See their images from early embryos acquired using the ApoTome.

Okamoto, I. et al. Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development. Nature. 472:370-4 (2011).
Drosophila larval neuromuscular junction Einat Peled and Ehud Isacoff used the LSM 5 LIVE to monitor real-time transmission of neurotransmitters from neurons to target cells in the model glutamatergic system of the Drosophila larval neuromuscular junction.

Peled, E.S. and E.Y. Isacoff. Optical quantal analysis of synaptic transmission in wild-type and rab3-mutant Drosophila motor axons. Nat Neurosci. 14:519-26 (2011).
Bone Remodeling Thorsten Schinke and colleagues investigated the role of Wnt receptors of the Frizzled family in regulation of bone remodeling. See images of von Kossa/van Gieson staining of bone sections acquired with the Axio Scope using an AxioCam.

Albers, J. et al. Control of bone formation by the serpentine receptor Frizzled-9. J Cell Biol. 192:1057-72 (2011).
Protein Domains in P. Falciparum Daniel Goldberg and colleagues examined the function of specific protein domains in P. falciparum, one of the parasites which causes malaria. Take a look at colocalization studies in P. falciparum using the Axio Scope.

Muralidharan, V. et al. Asparagine repeat function in a Plasmodium falciparum protein assessed via a regulatable fluorescent affinity tag. PNAS 108:4411-6 (2011).
AVE Cells Shankar Srinivas and colleagues investigated anterior visceral endodern (AVE) cells and their migration during mouse development. See 3D-renderings of mouse embryos in development using the LSM 710 in PLoS Biology.

Trichas, G. et al. Nodal dependent differential localisation of dishevelled-2 demarcates regions of differing cell behaviour in the visceral endoderm. PloS Biol. 9:e1001019 (2011).
House germinal centers See images of mouse germinal centers acquired using the Axio Scope in Bali Pulendran's recent paper where he and colleagues investigate how vaccines induce successful antibody responses.

Kasturi, S.P. et al. Programming the magnitude and persistence of antibody responses with innate immunity. Nature 470, 543-7 (2011).
Longevity in C. Elegans Andrew Dillin and colleagues at the Salk Institute investigate a mechanism for longevity in C. elegans. See their paper in Nature with images collected using an AxioCam and some with optical sectioning from theApoTome.

Mair, W. et al. Life extension induced by AMPK and calcineurin is mediated by CRTC-1 and CREB. Nature 470, 404-8 (2011).
EGFR Mien-Chie Hung and colleagues investigated regulation of EGFR, a cell surface receptor with multiple roles in cell fate determination, proliferation, cell migration and apoptosis. They used the LSM 710 to investigate localization of specific forms of EGFR in cultured cells.

Hsu, J.M. et al. Crosstalk between Arg 1175 methylation and Tyr 1173 phosphorylation negatively modulates EGFR-mediated ERK activation. Nat Cell Biol. 13, 174-81 (2011).
T cells and lymphoid tissue Gérard Eberl and colleagues examined certain types of T cells and lymphoid tissue and their effects on inflammatory bowel disease. See images of lymphoid tissue in mice colons acquired using the Axio Imager and processed with AxioVision software.

Lochner, M. et al. Microbiota-induced tertiary lymphoid tissues aggravate inflammatory disease in the absence of RORgamma t and LTi cells. J Exp Med. 203, 125-34 (2011).
Formation of spatial assymetries in neural connectivity in mice Botond Roska and colleagues investigated the formation of spatial asymmetries in neural connectivity in mice. See their images of mice retinas acquired with the LSM 700.

Yonehara, K. et al. Spatially asymmetric reorganization of inhibition establishes a motion-sensitive circuit. Nature 469, 401-10 (2011).
Dendritic cells Dendritic cells play a central role in the immune system and are critical in fighting infection. Boris Reizis and colleagues investigated different types of dendritic cells and how they develop in the body. See images of these cells acquired with the LSM 710.

Ghosh, H.S. et al. Continuous expression of the transcription factor e2-2 maintains the cell fate of mature plasmacytoid dendritic cells. Immunity 33, 905-16 (2010).
Steroid hormones Michael O’Connor and colleagues investigated steroid hormones that are responsible for regulating maturation of animals from juvenile to adult. Check out their images of Drosophila embryos acquired with the LSM 710 as they examine the effect of inactivating certain hormones during development.

Rewitz, K.F. et al. Steroid hormone inactivation is required during the juvenile-adult transition in Drosophila. Dev Cell. 19, 895-902 (2010).
Wnt signaling De Robertis and colleagues investigated Wnt signaling, a process that is integral for development, tissue regeneration, stem cells and cancer. See their paper which uses the Axio Imager equipped with an ApoTome to investigate colocalization in cultured cells.

Taelman V.F. et al. Wnt signaling requires sequestration of glycogen synthase kinase 3 inside multivesicular endosomes. Cell 143, 1136-48 (2010).
Time lapse imaging Montell and colleagues performed time lapse imaging experiments with the LSM 710 NLO to study how molecular dynamics control organ shape in the developing fly embryo.

He, L. et al. Tissue elongation requires oscillating contractions of a basal actomyosin network. Nat Cell Biol. 12, 1133-42 (2010).
Combining superresolution PAL-M with electron microscopy Jorgensen and colleagues report on methods for combining superresolution PAL-M with electron microscopy. They use these techniques to investigate histone and mithochondrial localization.

Watanabe, S. et al. Protein localization in electron micrographs using fluorescence microscopy. Nat Methods. 8, 80-4 (2010).


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